Chlorophyll content, pigment extraction
I have been looking for different methods to measure leaf chlorophyll content. And I have found a lot of different approaches: 1. using different solvents (acetone; dimethyl sulphoxide (DMSO); Methanol; ethanol); 2. by griding the samples with the solvents; making a powder out of the samples and then mix it with the solvent; or just making the extraction by leaving the sample in the solvent in a water bath at 65 celcius degrees; 3. using centrifuges or not; 4. using High Performance Liquid Chromatography (HPLC); 5. measuring with spectrophotometers; 6. using Arnon's (1949) equations for the chlorophyll content; or Porra's (1989) equations.
Does anyone have a recommendation about which methods is better?
Can somebody give me a reference that can give me a light about which approach is more accurate or reliable?
Thanks a lot for your help!