Mutations from ultraviolet light.
Does anyone know how much ultraviolet light is needed to make mutations in pollen? I have read of it being used, but I read it years ago in school. 25 years, and it is a bit fuzzy in my mind.
The reason I want to use ultraviolet light is that it makes point mutations without messing up the chromosomes.
And pollen is the only reproductive tissue that the untraviolet light will reach.
My purpose is to get a lycopene pink arilbred iris. The lycopene pink is recessive in tall bearded iris (autotetraploids) and in standard dwarf bearded iris (allotetradloids). The arilbreds are a different allotetaploid from the standard dwarf bearded, and no lycopene pinks exist in them.
My plan is to use arilbred pollen from arilbreds that have the lycopene gene in them from the tall bearded side of their ancestry. I'll irradiate the pollen with the light, then use it on the standeard dwarf bearded with lycopene. The mutations in the lycopene gene should give hybrids that show the lycopene color.
The arilbred x standard dwarf bearded F1 iris are sometimes partially fertile. This should get the mutation into the arilbred iris.
Any comments on this are welcome. Especially on how much and the type of ultraviolet light to use.