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doubling rhe chromosomes of rose seedlings

Posted by henry_kuska z5 OH (kuska@neo.rr.com) on
Thu, Jun 14, 12 at 11:56

"The technique used to double the chromosome number of C. japonica was applied to the three species mentioned to determine if it would be effective for inducing polyploidy and, if so, optimal duration of treatment. Seedlings were treated at the cotyledon stage for 0 (control), 10, 20, or 30 days with an aqueous solution containing 150 μM oryzalin + 0.1% Tween® 20 using a standard household spray bottle that created a fine mist."

See the "Abstract for research paper" link below.

If you are going to spray, I suggest a long sleeve shirt, long pants, face mask and double gloves (rubber and plastic). When I did this type of experiment, instead of spraying; I painted the solution on the buds (but I used the more dangerous DMSO instead of the safer Tween).

http://home.roadrunner.com/~kuska/chromosome.htm

Here is a link that might be useful: Abstract for research paper


Follow-Up Postings:

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RE: doubling rhe chromosomes of rose seedlings

  • Posted by seil z6b MI (My Page) on
    Tue, Aug 21, 12 at 18:39

I think I'll just take my chances. I'm not fond of using sprays for anything.


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