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Fri, Jun 5, 09 at 23:13
| Did not know if anyone has had experience converting diploid
salvias into tetraploids?I am aware of the chemicals/process used to do this but wondered if it has been attempted or not. Art |
Follow-Up Postings:
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- Posted by wantonamara 8bTx (My Page) on Sat, Jun 6, 09 at 19:38
| I didn't understand anything in that sentence. |
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- Posted by rich_dufresne z7 NC (My Page) on Sat, Jun 6, 09 at 22:45
| By treating cuttings with a colchicine mixture at just the right concentration, half of them will root, while the others die from poisoning. Those that make it have had their cytoplasm modified, giving rise to chromosome doubling. These cuttings are set out, and the axillary growth observed for unusual shoots, which are cut off and rooted. These will become the tetraploid children. Or at least, that's how I remember it. It may be possible to apply the colchicine to the incipient axillary shoots at the nodes, and there are certain herbicides that are more accessible than colchicine that will do the same at subleathal doses. |
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